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TaKaRa tb green premix ex taq
Tb Green Premix Ex Taq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tb+green+premix+ex+taq/TB+Green+Premix+Ex+Taq/pm42888933-277-16-21
Average 99 stars, based on 11140 article reviews

tb green premix ex taq - by Bioz Stars, 2026-11

99/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Bone Marrow Macrophage STING Signaling is a Direct Nexus Linking Marrow Adipose Expansion to NAD + Depletion and Osteogenic Decline in Skeletal Aging
Article Snippet: Total RNA was extracted from cells using TRNzol Universal (TIANGEN, DP424) and reverse‐transcribed into cDNA using the PrimeScript RT Master Mix (TaKaRa, RR036A). .. Subsequent qPCR was performed on a LightCycler 96 system using TB Green Premix Ex Taq (TaKaRa, RR820A). ..

Article Title: A HAT7/ATHB13/Brassinosteroid signaling module mediates growth of laticifer cells in Euphorbia lathyris.
Article Snippet: .. 24 Real-time PCR was performed using an ABI 7500 Fast Real-Time Sequence Detection System and 25 TB Green Premix Ex Taq (Takara Bio Inc.). ..

Article Title: Novel phorbol-12-O-octoate derivatives exert a dual effect by inhibiting de novo HIV-1 infection and reactivating latent virus.
Article Snippet: EGR1 mRNA expression levels were measured after stimulating ACH2 cells with 1 μM HPB-5073 for 1 h and 24 h. Total RNA was extracted from the treated cells using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) and reverse transcribed using the PrimeScript Fast RT reagent Kit with gDNA Eraser (TaKaRa, Kusatsu, Japan). .. Relative gene expression levels were quantified by RT-qPCR using TB Green Premix Ex Taq or Premix Ex Taq (Probe qPCR) (TaKaRa). ..

Sequencing:

Lysis:

Article Title: PTTG1 drives intestinal-type gastric cancer progression via G2/M checkpoint-mediated cell-cycle reprogramming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-PTTG1 antibody CST Cat# 13445T anti-Ki67 antibody CST Cat# 9027S anti-Cyclin B1 antibody CST Cat# 12231S anti-p-CDK1 antibody CST Cat# 4539T anti-MUC2 antibody Abcam Cat# ab272692; RRID: AB_2888616 anti-γH2AX antibody CST Cat# 9718S anti-E2F1 antibody CST Cat# 3742S; RRID: AB_2096936 anti-β-ACTIN antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 Alexa 594 donkey anti-rabbit secondary antibodies Thermo Fisher Scientific Cat# A11012; RRID: AB_141359 Alexa 488 donkey anti-rabbit secondary antibodies Thermo Fisher Scientific Cat# A32731; RRID: AB_2633280 Alexa 647 donkey anti-rabbit secondary antibodies Thermo Fisher Scientific Cat# A32733; RRID: AB_2633282 DAPI Thermo Fisher Scientific Cat# 62248 Goat-anti-mouse IgG Jackson ImmunoResearch Cat# 115-035-166; RRID: AB_2338511 Goat-anti-rabbit IgG Jackson ImmunoResearch Cat# 111-035-144; RRID: AB_2307391 HRP-conjugated anti-rabbit secondary antibodies ZSGB-Bio Cat# PV-6001; RRID: AB_2864333 Bacterial and virus strains siNC RiboBio N/A siPTTG1#1 (5′-3′): GAAGACUGUUAAAGCAAAA RiboBio N/A siPTTG1#2 (5′-3′): GAUGAUGCCUAUCCAGAAA RiboBio N/A shNC Shanghai Genechem N/A shPTTG1#1 (5′-3′): GAAGACUGUUAAAGCAAAA Shanghai Genechem N/A shPTTG1#2 (5′-3′): GAUGAUGCCUAUCCAGAAA Shanghai Genechem N/A PTTG1 overexpression Shanghai Genechem N/A Biological samples Human gastric tissue samples The Affiliated Cancer Hospital of Nanjing Medical University, The First Affiliated Hospital of Nanjing Medical University This study Human gastric organoid The First Affiliated Hospital of Nanjing Medical University This study Nude mice tissue samples the Experimental Animal Center of Nanjing Medical University This study Chemicals, peptides, and recombinant proteins sCelLiveTM Tissue Preservation Solution Singleron Cat# 1010012 sCelLiveTM Tissue Dissociation Solution Singleron Cat# 1020012 GEXSCOPE red blood cell lysis buffer Singleron Cat# 1200050002 TrypLETM Express Enzyme Invitrogen Cat# 12605010 TRIzolTM Reagent Invitrogen Cat# 15596018 PrimeScriptTM RT Master Mix TaKaRa Cat# RR036A (Continued on next page) iScience 29, 117742, November 20, 2026 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER TB Green Premix Ex Taq TaKaRa Cat# RR420A RIPA lysis buffer Beyotime Cat# P0013J phosphatase inhibitor cocktail Bimake Cat# B15002 protease inhibitor cocktail MedChemExpress Cat# HY-K0010-1ML Chemiluminescent HRP Substrate Merck Millipore Cat# WBKLS0500 Cultrex Organoid Harvesting Solution Trevigen Cat# 3700-100-01 Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix Corning Cat# 356231 LipofectamineTM RNAiMAX Transfection Reagent Invitrogen Cat# 13778150 RPMI1640 BI Cat# 01-100-1ACS Human Gastric Epithelial Organoid Kit bioGenus Cat# K2004-HG Critical commercial assays Cell Counting Kit-8 Dojindo Cat# CK04 Cell LightTM EdU DNA Cell Proliferation Kit RiboBio Cat# C10310-1 (PI)/RNase staining solution Dojindo Cat# C543 Alcian Blue-Periodic acid-Schiff Stain Kit Solarbio Cat# G1285-6*50mL Hyperactive pG-MNase CUT&RUN Assay Kit for PCR/qPCR Vazyme Cat# HD101-01 Deposited data Cell lines RNA-seq analysis This paper HRA015866 https://ngdc.cncb.ac.cn/gsa-human/browse/ HRA015866 Gastric tissue samples scRNA-seq analysis This paper HRA015134 https://ngdc.cncb.ac.cn/gsa-human/browse/ HRA015134 The Cancer Genome Archive (TCGA) RNA-seq data UCSC Xena TCGA-STAD Asian Cancer Research Group (ACRG) RNAseq data GEO GSE66229 NJGC RNA-seq data GSA-Human HRA008551 Experimental models: Cell lines MKN1 (RRID: CVCL_1415) the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences N/A MKN7 (RRID: CVCL_1417) the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains BALB/c nude mice GemPharmatech Co., Ltd (Nanjing, China) N/A Oligonucleotides ..

Protease Inhibitor:

Membrane:

Transfection:

CCK-8 Assay:

Staining:

CUT&RUN:

Polymerase Chain Reaction:

RNA Sequencing:

Analysis:

Tissue:

Cancer Research:

Cell Biology:

Amplification:

Article Title: PI3KC3/BECLIN1-dependent lipophagy mediates phosphorus-induced reduction of hepatic lipid deposition in hybrid yellow catfish.
Article Snippet: To investigate the molecular mechanism by which phosphorus (Pi) regulates hepatic lipid deposition in hybrid yellow catfish (Pelteobagrus fulvidraco × Pelteobagrus vachelli), a 56-day in vivo feeding trial was conducted with five dietary Pi levels: 0.54%, 0.65%, 0.74%, 0.98%, and 1.28%.. Combined with in vitro incubation experiments using primary hepatocytes, this study focused on the PI3KC3/BECLIN1-mediated lipophagy pathway to assess hepatic morphology, expression of autophagy-related genes/ proteins, and lipophagic activity.. The results showed that hepatic morphology was intact in fish fed 0.65%– 0.74% dietary Pi, whereas obvious vacuolization and lipid accumulation were observed in the 0.54% and 1.28% Pi groups.

Quantitative RT-PCR:

Article Title: Age-Dependent Flowering Transition in Cymbidium goeringii: Physiological Characterization and Small RNA Profiling.
Article Snippet: First-strand cDNA was synthesized from 1 μg of total RNA using the miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (MR101-01, Vazyme, Nanjing, China) according to the manufacturer’s instructions. .. qRT-PCR was performed using TB Green Premix Ex Taq (RR420, Takara, Kyoto, Japan) in a 20 μL reaction volume. ..

Article Title: Inhibition of miR-125b-1-3p alleviates acute kidney injury and mitochondrial membrane potential loss by targeting GSTK1.
Article Snippet: As a type of regulated necrosis driven by iron-dependent lipid peroxidation, ferroptosis critically contributes to acute kidney injury (AKI).. Currently, whether miR-125b-1-3p is engaged in this pathological process remains unknown.. Here, miR-125b-1-3p was upregulated in kidney tissues from an AKI model (GSE125305), in serum from a small exploratory cohort (15 AKI patients vs 15 controls), and in hypoxia/reoxygenation (H/R)-treated NRK-52E cells and erastin-induced ferroptosis models (n = 3).

Gene Expression:

other:

Article Title: STING engages PKR to drive lysosomal cell death in lung ischemia-reperfusion injury.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Cy3 Beyotime Cat# A0521; RRID: AB_2923334 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, FITC Beyotime Cat# A0562; RRID: AB_2923335 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Cy3 Beyotime Cat# A0516; RRID: AB_2893015 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Beyotime Cat# A0473; RRID: AB_2891322 Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Cy3 Beyotime Cat# A0502; RRID: AB_3065047 InVivoSIM anti-human IFNAR1 (Anifrolumab Biosimilar) BioXcell Cat# SIM0022; RRID: AB_3696361 Chemicals, Peptides, and Recombinant Proteins Phosphatase inhibitor cocktail Sigma-Aldrich Cat# P5726 Methyl Cellulose Sigma-Aldrich Cat# M0512 Protease inhibitor cocktail Sigma-Aldrich Cat# P8340 Lipofectamine 2000 Reagent ThermoFisher Scientific Cat# L11668019 Lipofectamine 3000 Reagent ThermoFisher Scientific Cat# L3000001 Lipo8000 Transfection Reagent Beyotime Cat# C0533 2’3′-cGAMP Invivogen Cat# tlrl-nacga23-02 DNase I Roche Cat# 9003-98-9 Collagenase Type I ThermoFisher Scientific Cat# 17100017 Dispase Roche Cat# 40104ES80 Palbociclib MedChemExpress Cat# HY-50767 Amlexanox MedChemExpress Cat# HY-B0713 BAY 11-7082 MedChemExpress Cat# HY-13453 2’,3’-dideoxycytidine MedChemExpress Cat# HY-17392 GSK2606414 MedChemExpress Cat# HY-18072 Brefeldin A MedChemExpress Cat# HY-16592 5Z-7-Oxozeaenol MedChemExpress Cat#HY-12686 TUDCA MedChemExpress Cat#HY-19696 LLOMe MedChemExpress Cat#HY-129905 Bafilomycin A1 MedChemExpress Cat#HY-100558 E64d MedChemExpress Cat#HY-100229 CA-074Me MedChemExpress Cat#HY-100350 Z-VAD-FMK MedChemExpress Cat#HY-16658B Ferrostatin-1 MedChemExpress Cat#HY-100579 Recombinant Human IFN-β Peprotech Cat#300-02BC Triton X-100 Sigma-Aldrich Cat#T8787 Digitonin Sigma-Aldrich Cat# 300410 Cls IV Sigma-Aldrich Cat# C4BIOC Anti-FLAG M2 Affinity Gel Sigma-Aldrich Cat# A2220 Critical Commercial Assay TRIzol Reagent ThermoFisher Scientific Cat# 15596-018 TB Green Premix Ex Taq Takara Cat# RR420B Pierce Co-immunoprecipitation kit ThermoFisher Scientific Cat# 26149 (Continued on next page) ll Article Molecular Cell 86, 1–19.e1–e11, November 5, 2026 e2

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